Fully automatic colony counter analyzer

It adopts an all-metal chassis with no ambient light interference. The multi-mode lighting system combines fog diffuse reflection lighting, white translucent bottom lighting, and compound suspended dark field lighting to adapt to various types of plate imaging.

The software is equipped with rich image recognition algorithms and is suitable for complex colony image processing. The new version of the software adds a fast statistics module. Customers only need to rotate the mouse wheel to easily obtain the best statistical results, eliminating the hard work of manual parameter setting and experimentation.

Fog diffuse lighting

The fog light system composed of 96 LED arrays and nano-light reflective materials adopts an embedded design. High-bright, foggy diffuse reflected light fills the photography cabin, forming uniform upper illumination. The colonies and culture medium have natural colors, and the glass petri dishes will not form common refraction phenomena such as light spots and halos.

Duplex suspended dark field lighting

Duplex dark field lighting consists of double layers of dark field contour light and a black background, and the light intensity can be adjusted freely. The uniform, parallel mixed retroreflected light penetrates the culture medium and gives the colonies a beautiful outline, forming bright colonies on a dark background.

White translucent bottom lighting

Bottom lighting is composed of a white LED backlight module light source, a reflective plate, and a light guide plate, which can control the intensity and direction of the transmitted light. Instead of conventional backlighting that can only form silhouettes, it has a more three-dimensional expression for light colors and tiny colonies.

Efficient digital imaging

High-fidelity fixed-focus lens and 8.5-megapixel USB3.0 high-speed CMOS industrial camera can quickly display the colony shape and color.

Intelligent fast statistics

Seven quick statistics buttons are simple and easy to use. Just rotate the mouse wheel to achieve continuous parameter changes and easily obtain the best statistical results.

Eight complex statistical algorithms adapt to the diversity and complexity of plates, such as multiple adhesion, uneven culture medium, coexistence of impurities and colonies, pigment diffusion, close color of colonies and culture medium...

Determination of total bacterial count

Nutrient agar, common bacterial colony characteristics: small colonies, neat edges, smooth surface, mostly round and oval

Coliform count

Crystal violet neutral red bile salt agar (VRBA colony characteristics: purple-red, with a red bile salt precipitation ring around the colony, about 0.5 mm in diameter

Mold and Yeast Counts

Bengal red medium, common mold colony characteristics: larger colonies, loose and fluffy texture, darker color in the middle, and divergent edges

3M bacterial count test strips

The bacterial metabolites undergo a redox reaction with the indicator TTC , and the colonies are small and red.

Main functions and technical indicators

1. Lighting system

l Fully enclosed steel and aluminum alloy chassis (32×34×46cm): precise and sturdy, ensuring light sealing

l Plate loading cabin: Aluminum alloy frame, drop-down partition window, eliminates interference from ambient stray light, blocks UV leakage, and prevents dust from entering

Backlighting

High-density white LED array creates uniform, bright white transmitted light, ensuring uniform illumination of the edges and middle of the culture dish

Duplex suspended dark field lighting

White LED and blue LED are intertwined and mixed, broadband retroreflection, forming a cosmic blue background

Fog diffuse lighting

1）. 96 LED arrays and nano-light reflective materials form an embedded fog light system with 360° continuous diffuse reflection to highlight the color of the colonies.

The color and texture can be improved, and the spots and halos formed by the refraction of the glass petri dish can be eliminated.

2）. Color temperature range: 3100K-5800K Illuminance range 50--7000 Lux

3）. LED lifespan ≧20000 hr

l UV light source: 254nm for cavity disinfection and UV mutagenesis

Light source controller

1）. Invisible pop-up control panel, 5-way lighting selection switch, 4-channel stepless brightness adjustment, dual-channel color temperature adjustment

2）. Can freely switch and choose single mode lighting or combined mode lighting

2. Digital imaging

•  Standard definition industrial fixed focus lens: 8mm,3.0 mega-pixel, 1/2", Distortion <1%, F1.4～F32, C-Mount
•  Professional CMOS camera: chip size 1/2.4"; CMOS physical pixels 8.5 million, 3328x2548 ; single pixel ruler inch1.67x1.67µm

3. Colony analysis module

1. Rapid colony counting

l Roller parameter adjustment statistics (4 types): homogeneous plate, uneven background, tiny colonies, colored background

l One-click response statistics (3 types): single color statistics, mold statistics, trans statistics

2. Advanced colony statistics

• Dynamically adjust statistics: Statistical results can be dynamically adjusted and corrected to quickly obtain the best statistical results.
• Deviation estimation statistics: suitable for situations where colonies have many colors and are complex.
• Level set multi-model algorithm: search operation to obtain the best image segmentation effect and adapt to medium background transformation
• Specific colony statistics: identify specific colonies based on colony color, size, and outline characteristics
• Trans statistics: suitable for extremely complex colony types and uniform culture medium background
• Removal of miscellaneous bacteria and impurities: automatic removal of miscellaneous bacteria and impurities based on differences in shape, size, and color

3. Basic colony counting function

• Petri dish type: pouring, coating, membrane filtration, 3M paper
• Full plate colony statistics: total number of colonies are counted and displayed according to 25 size categories.
• Area selection statistics: You can choose circular, rectangular, or any circled area for statistics. ØMulti -domain parallel statistics: One-time multi-region synchronization statistics; multi-region "hollow" statistics
• Diameter classification statistics: set the diameter range and count colonies of a specific size
• Mouse click statistics: quickly mark and add colonies, suitable for counting colonies on the edge of the petri dish
• Colony adhesion segmentation: Automatically segment colonies that adhere to each other. The user can choose to segment or not segment chain-shaped colonies.

4. Mesh filter membrane and 3M test piece

• Black solid line grid one-click statistics
• 3M total bacteria test strips, 3M Staphylococcus aureus test strips: one-click statistics
• 3M coliform test strips, 3M E. coli/coliform rapid test strips: one-click statistics + manual selection

5. Advanced Tools

• Grid Clear: Eliminate filter grid background interference
• Manual counting correction: adding or removing colonies
• Exclude contaminated areas: Use the mouse to outline any contaminated area and automatically eliminate the number of colonies in the contaminated area.
• Background text removal: Automatically eliminate marker interference
• Manual adhesion segmentation: manual segmentation of multiple adherent colonies
• Automatic parameter conversion: Petri dish diameter and sample dilution input to achieve automatic conversion

6. Calibration and measurement

• Instrument calibration: instrument comes with calibration, manual correction calibration
• One-click rapid measurement: One-click determination of large colonies, suitable for single colony analysis of fungi and actinomycetes.
• Automatic measurement of the whole dish: Equivalent diameter, area, long and short diameter, circumference and roundness analysis of the whole dish colony
• Multi-directional ruler measurement, manual accurate measurement: length, angle, radian, area, arc, arbitrary curve

4. Data Security and Management

• "Management, operation, review" multiple structures with separate functions and authorities to ensure data security, integrity and authenticity
• Single dish data record: measured number of colonies, number of colonies converted by area, number of colonies converted by dilution, morphological parameters of each colony, size classification
• Level statistics, regional statistics
• Electronic data records: sample source, serial number, dilution, plate pictures, identification results, count values, statistical tools used, parameters
• Number settings and corrections
• Electronic data is automatically stored or printed out in PDF or Excel format

5. Instrument specifications and configuration

• 1 CA-IC-330 host
• Colony analysis software
• Brand business desktop computer: dual-core CPU/4G memory/1T hard drive/21.5" high-definition screen, Windows 10 system