Gene Transduction Device

GG-1000

The gene gun technology (Particle Bombardment) is a new gene transfer technology that accelerates micron-sized gold or tungsten particles coated with nucleic acids to the desired rate to introduce genes into cells, tissues or organs. Get instantaneous expression of stability and high efficiency, transformation. With fast, easy, safe and efficient features.

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Product info

Description:
The gene gun technology (Particle Bombardment) is a new gene transfer technology that accelerates micron-sized gold or tungsten particles coated with nucleic acids to the desired rate to introduce genes into cells, tissues or organs. Get instantaneous expression of stability and high efficiency, transformation. With fast, easy, safe and efficient features.

Performance characteristics

  1. simple, fast, flexible function, a variety of cell types common
  2. contribute to transient expression and stable expression
  3. only a small amount of DNA and cells, without vector DNA
  4. to be able to carry out multiple plasmid co-introduction of large fragments of DNA unlimited
  5. in vivo and in vitro transformation can be high specificity
  6. bombardment room unique circular conversion room, removable, easy to clean and disinfect
  7. low cost of supplies, each gun except high-pressure gas costs about 18 yuan
  8. high repeatability
  9. other methods can not be completed, there are special needs of cell growth transformation
  10. the particles are evenly distributed
  11. the maximum control of the experimental process

Application areas

  1.  animals: tissue (skin, organs), cells, organs and so on
  2. plants: living plants, leaves or other plant tissues and organs
  3. yeast, bacteria, other microorganisms
  4. gene expression research
  5. agricultural biotechnology and crop improvement
  6. plant vaccine

Patent No .: ZL011 05512.X200630105830.7

Technical parameters


Experimental conditions

In vitro, from the body, plants (in vivo)

Target region

(big)40cm2

Pressure range

3-12MPa

Target Type

Animals: cell and organ culture
Plants: The smaller of the whole plants, cell culture, explant, yeast, bacteria and other micro-organelles, chloroplast, mitochondria

Voltage

220V/110V

Gas

Helium or nitrogen ( helium is more otimized)

Metal Particle

Φ0.6-1.6μm,(Common Φ1μm)

Standard configuration:

  1. Host
  2. vacuum pump
  3. 1000 gun supplies (3.5 / 4.5 / 7Mpa rupturable membrane, carrier membrane, stainless steel mat net, 2 bottles of powder, tungsten powder 1 bottle);

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